Biomedical Engineering Reference
In-Depth Information
Fig. 4.5
BMP2 fused to artificial proteins containing titanium binding peptides were immobilized
on titanium surface. Artificial proteins could associate with titanium surface in a reversible manner,
enabling them to be BMP2 with an active state. Reprinted with permission from [
89
]. (Copyright
2009, Elsevier)
titanium surface using the artificial protein. Indeed, Kashiwagi et al. observed BMP-
induced differentiation of osteoblastic cells when myoblastic cells were cultivated
on a BMP2-modified titanium surface (Fig.
4.5
,[
89
]).
Tsuji et al. created an artificial protein by combining DMP1-derived peptides and
a titanium-binding peptide. The resulting protein showed bifunctionality—titanium
binding activity and nucleation activity of crystalline calcium phosphate-, and could
be used for coating of a titanium surface with OCP film under biologically relevant
conditions [
90
]. The use of OCP coated titanium for implants would accelerate
adhesion between the implants and surrounding bone.
4.8
Closing Remarks
In this chapter, we summarized the results of various research studies pertaining
to biomineralization related to HAP crystal formation from the viewpoint of
protein science and protein engineering. The utilization of protein and peptide
engineering technology should make it possible to control biomineralization at the
molecular as well as at the cellular level. Additional proteins with the capability of
controlling or affecting biomineralization will likely be constructed. Understanding
