Biomedical Engineering Reference
In-Depth Information
by the dynamic actin cytoskeleton. The experimental setup described here for
observation of keratocytes by phase contrast microscopy enables detection of cell
peripheral dynamics in a whole cell with sub-micrometer spatial resolution and a
sub-second temporal resolution for an observation duration of minutes. The hierar-
chical properties can be extracted by varying the level of coarse graining of the
quantitative data contained in a sequence of images. The multiscale data demon-
strates a spatiotemporally nested pattern of travelling waves characterized by local
protruding domains embedded in a global protruding region. The top-down approach
for multiscale analysis based on high-resolution and wide-fi eld live cell imaging
data can clarify the characteristics of hierarchically coordinated cellular protrusion
resulting from an actin cytoskeletal system (Pollard and Cooper
2009
; Ponti et al.
2004
) that involves structurally and kinetically different actin networks.
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