Biomedical Engineering Reference
In-Depth Information
0.01
a
Calyculin
Control
Blebbistatin
0.00
-0.01
*
*
-0.02
*
*
*
*
*
*
-0.03
x
Mean ± S.D.,
n
= 9
*
*
p
< 0.05 vs. control (t-test)
-0.04
5.4
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0.0
0.01
b
0.00
*
*
-0.01
*
*
*
*
-0.02
5.4
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0.0
0.00
c
-0.04
Blebbistatin
-0.08
Control
Calyculin
-0.12
5.4
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0.0
Distance from the leading edge,
x
[
µ
m]
Fig. 5.4
Distribution of mean strain rate in the
a
ctin network under pertu
rb
ation (Okeyo et al.
2009
). Dist
ri
butions of (
a
) parallel strain rate,
e
p
, (
b
) normal strain rate,
e
n
, (
c
) total parallel
strain rate,
e
p
total
, in the lamellipodia of migrating keratocytes under actomyosin perturbation
(Adapted with permission from Elsevier Ltd.: [Journal of Biomechanics], copyright (2009))
higher for calyculin than for control (Fig.
5.4a
). Moreover, strain rate values in this
case consistently increases toward the back of the lamellipodia, suggesting that the
F-actin network is under a state of increasing compressio
n
toward the back of the
lamellipodia, as described earlier. Indeed, the value of
e
p
at the leading edge
