Biomedical Engineering Reference
In-Depth Information
a
b
Actin
Myosin II
Merged
80
SF
60
40
20
Actin
Myosin II
0
10.0
8.0
6.0
4.0
2.0
0.0
Distance from the leading edge [mm]
c
d
80
60
SF
40
20
0
10.0
8.0
6.0
4.0
2.0
0.0
Distance from the leading edge (mm)
e
f
80
60
40
20
0
10.0
Distance from the leading edge [mm]
8.0
6.0
4.0
2.0
0.0
Fig. 5.1
Actomyosin distribution in the lamellipodia of migrating fish keratocytes (Okeyo et al.
2009
). (
a
,
c
,
e
) Fluorescent images of actin (
left panel
), myosin II (
middle panel
) and merge (
right
panel
) for calyculin-treated (
a
), control (
c
), and blebbistatin-treated (
e
) acquired by laser scanning
microscopy of stained keratocytes. (
b
,
d
,
f
) Distribution profiles of F-actin and myosin II densities
over the
white rectangular boxes
shown in the respective merged images. The
arrows
marked SF
in (
b
) and (
d
) indicate the intensity rise due to the presence of stress fibers. Scale bar is 5 μm
(Adapted with permission from Elsevier Ltd.: [Journal of Biomechanics], copyright (2009))
