Biomedical Engineering Reference
In-Depth Information
work in concert to regulate the molecular processes of polymerization and
depolymerization. Moreover, we have reviewed mechanical forces involved in
cell motility, with particular attention on active force generation by actomyosin
interactions in the lamellipodia. And elucidated how the associated contractile forces
infl uence actin network dynamics. Furthermore, we have correlated intracellular
forces with traction forces through the molecular clutch mechanism, and high-
lighted that adhesion to the ECM is essential to the structural integrity of the actin
cytoskeleton, particularly stress fi bers.
Overall, we have detailed the various mechanisms for the regulation of actin
dynamics. In Chap.
3
, we investigate the quantitative details of actin network dynamics
using fl ow mapping techniques, and we quantitatively examine the implication of
coupling between actin network fl ow and turnover for cell migration.
References
Akin O, Mullins RD (2008) Capping protein increases the rate of actin-based motility by promoting
fi lament nucleation by the Arp2/3 complex. Cell 133(5):841-851
Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2008) The cytoskeleton. In: Molecular
biology of the cell, 5th edn. Garland Science, New York, pp 965-1052
Ananthakrishnan R, Ehrlicher A (2007) The forces behind cell movement. Int J Biol Sci 3(5):303-317
Anderson KI, Wang YL, Small JV (1996) Coordination of protrusion and translocation of the kera-
tocyte involves rolling of the cell body. J Cell Biol 134(5):1209-1218
Andrianantoandro E, Pollard TD (2006) Mechanism of actin fi lament turnover by severing and
nucleation at different concentrations of ADF/cofi lin. Mol Cell 24(1):13-23. doi:
10.1016/j.
Aratyn-Schaus Y, Gardel ML (2008) Biophysics: clutch dynamics. Science 322(5908):1646-1647.
doi:
322/5908/1646 [pii] 10.1126/science.1168102
Arora PD, McCulloch CAG (1996) Dependence of fi broblast migration on actin severing activity
of gelsolin. J Biol Chem 271(34):20516-20523
Borisy GG, Svitkina TM (2000) Actin machinery: pushing the envelope. Curr Opin Cell Biol
12(1):104-112
Buckley CD, Ed Rainger G, Bradfi eld PF, Nash GB, Simmons DL (1998) Cell adhesion: more than
just glue (review). Mol Membr Biol 15(4):167-176
Burridge K, Molony L, Kelly T (1987) Adhesion plaques: sites of transmembrane interaction
between the extracellular matrix and the actin cytoskeleton. J Cell Sci Suppl 8:211-229
Burton K, Park JH, Taylor DL (1999) Keratocytes generate traction forces in two phases. Mol Biol
Cell 10(11):3745-3769
Capaldo CT, Nusrat A (2009) Cytokine regulation of tight junctions. Biochim Biophys Acta
Biomembr 1788(4):864-871
Carlier MF, Laurent V, Santolini J, Melki R, Didry D, Xia GX, Hong Y, Chua NH, Pantaloni D
(1997) Actin depolymerizing factor (ADF/cofi lin) enhances the rate of fi lament turnover:
implication in actin-based motility. J Cell Biol 136(6):1307-1322
Chan CE, Odde DJ (2008) Traction dynamics of fi lopodia on compliant substrates. Science
322(5908):1687-1691
Chen CS (2008) Mechanotransduction - a fi eld pulling together? J Cell Sci 121(20):3285-3292
Choquet D, Felsenfeld DP, Sheetz MP (1997) Extracellular matrix rigidity causes strengthening of
integrin-cytoskeleton linkages. Cell 88(1):39-48
ChrzanowskaWodnicka M, Burridge K (1996) Rho-stimulated contractility drives the formation of
stress fi bers and focal adhesions. J Cell Biol 133(6):1403-1415. doi
:
10.1083/jcb.133.6.1403
