Biomedical Engineering Reference
In-Depth Information
a
90
60
30
Repulsion
Attraction
Threshold
w
s
= 5
µ
m
0
0
1
2
3
4
5
Groove width,
w
(
µ
m)
Repulsion
Attraction
Threshold
b
c
90
90
60
60
30
30
Repulsion
Attraction
s
w
w
= 1
µ
m
0
0
0
5 10 15
Groove spacing,
s
(
µ
m)
4
Groove width,
w
(
µ
m)
Fig. 10.7
Cell repulsion/attraction properties of grooves (Miyoshi et al.
2010
). (
a
) Result obtained
with various groove widths
w
at different angles of approach
θ
. Groove spacing is fixed (
s
= 5 ʼm).
(
b
) Result with various groove spacings
s
at different angles of approach
θ
. Groove width is fixed
(
w
= 1 ʼm). (
c
) Result with the single line groove of
w
= 4 ʼm at different angles of approach
θ
. The
threshold angle of approach shown in (
a
) and (
c
) is estimated by the ROC analysis (Adapted with
permission from Elsevier Ltd.: [Biomaterials], copyright (2012))
From the viewpoint of the motility-based cell filtering, cell turning behavior at the
boundary corresponds to cell repulsion by the grooved surface, and crossing the
boundary corresponds to cell attraction. In Fig.
10.7a
, the results from the analysis of
the behavior of individual cells at the boundary (being repelled or attracted) are plot-
ted with the vertical axis as the angle of approach
θ
and the horizontal axis as the
groove width
w
. As can be predicted from the qualitative cell trajectory data in
