Biomedical Engineering Reference
In-Depth Information
a
Intersecting grooves
Flat surface
Gr
o
ove
w
idth,
w
Groove depth,
20
µ
m
Gooved surface
Groove spacing,
s
b
Single line groove
Groove width,
w
Groove depth,
20
µ
m
Fig. 10.2
Model substrates with microgrooved structure for cell migration assay (Miyoshi et al.
2010
). (
a
) Schematic of the substrate consisting of a flat surface and an intersecting grooved sur-
face. The variables
w
and
s
stand for groove width and groove spacing, respectively. (
b
) Schematic
of the substrate with a single line groove. The variable
w
stands for groove width (Adapted with
permission from Elsevier Ltd.: [Biomaterials], copyright (2012))
Fig. 10.3
SEM images of the silicon substrates for the cell migration analysis (Miyoshi et al.
2010
). Intersecting grooves of (
a
)
w
= 1 ʼm,
s
= 5 ʼm; (
b
)
w
= 3 ʼm,
s
= 5 ʼm; and (
c
)
w
= 4 ʼm,
s
= 5 ʼm. Groove depth was 20 ʼm for all substrates. The fabricated samples were observed
with a scanning electron microscope. Original magnifications are: (
a
) and (
b
) 1,000 ×, and (
c
)
800×. All scale bars are 10 ʼm. Adapted with permission from Elsevier Ltd.: [Biomaterials],
copyright (2012)
