Biomedical Engineering Reference
In-Depth Information
of actin cytoskeleton along the edge of fi bronectin pattern can be identifi ed in
Fig.
8.4a
. Contrary to the typically observed meshwork appearance, the actin
cytoskeleton of the stationary cells in the fi gure lacks discrete meshwork lattice
which are known to be formed in fi sh keratocytes on an unpatterned surface (Okeyo
et al.
2009
; Verkhovsky et al.
1999
). This suggests that adhesion is necessary for the
formation of such a meshwork.
Figure
8.4b
shows the results of myosin II labelling by immunostaining for a
keratocyte attached on a fi bronectin micropattern. It can be noticed that myosin II
spots are evenly distributed over the fi bronectin micropattern, but do not show
Fig. 8.4
Fluorescence microscopic images of the actomyosin cytoskeleton and focal adhesion in
calyculin treated and untreated fi sh keratocytes (Okeyo et al.
2011
). The size of the rectangular
fi bronectin patterns is 18
m. (
a
) Actin cytoskeleton in a cell immobilized on a fi bronectin
micropattern. Actin cytoskeleton organization lacks the meshwork lattice that is typically observed
in keratocyte on an unpatterned surface. (
b
) Myosin II and (
c
) vinculin distributions in an immobi-
lized cell. (
d
) Actin cytoskeleton in a keratocyte undergoing protrusion across adhesion-suppressed
gap following actomyosin activation. (
e
) Myosin II and (
f
) vinculin distributions in a cell migrating
across adhesion suppressed gap. In this case, both myosin II and vinculin distributions co-localized
with that of actin cytoskeleton (Adapted with permission from Springer, Part of Springer
Science + Business Media: [Cellular and Molecular Bioengineering], copyright (2011))
ʼ
m × 38
ʼ
