Biomedical Engineering Reference
In-Depth Information
Fig. 8.2
Cell protrusion on a micropattern (Okeyo et al.
2011
). (
a
) Fluorescent image of a fi bronectin
pattern for modulating lamellipodial protrusion. (
b
) Phase contrast image of a keratocyte immobi-
lized on the micropattern shown in (
a
). Lamellipodial ruffl ing can be observed along the edge
of the pattern. (
c
) Illustration of defi nition of maximum lamellipodial length,
l
. (
d
) Distribution
of lamellipodial length,
l
, for cells immobilized on fi bronectin micropatterns in the presence
and absence of calyculin. The bell shape distribution shifts to the right after calyculin treatment,
implying an overall increase in lamellipodial length. Inset is a bar graph highlighting the increase
in the mean lamellipodial length after calyculin treatment. Scale bar is 10
m (Adapted with per-
mission from Springer, Part of Springer Science + Business Media: [Cellular and Molecular
Bioengineering], copyright (2011))
ΚΌ
cycles of protrusion and retraction. Since protrusion of the lamellipodia occurs on
an adhesion suppressed region, the extended length of the lamellipodia does not
anchor to the substrate and therefore retracts when, for instance, actomyosin
contractility causes cell contraction. Alternatively, instability resulting from the
