Biomedical Engineering Reference
In-Depth Information
Fig. 7.6
Representative example of the relationship between cell peripheral activity and cell periph-
eral shape (Miyoshi and Adachi
2012
). The result for a full-moon-shaped keratocyte cultured at
20 ﾰC. (
a
) Time sequence of phase contrast images showing the cell peripheral movement of a full-
moon-shaped keratocyte. Bar, 10 ʼm. Numbers in
upper left
corner indicate time in seconds. (
b
)
Multiscale nested structure of spatiotemporal pattern of the cell protrusion rate. The protrusion rates
are calculated at different time intervals
ʔt
: (
left
)
ʔt
= 13.58 s, (
right
)
ʔt
=2.72 s, and they are
mapped against space
ʸ
and time
t
. The ordinate time scale of the
right
map is enlarged five times
relative to that of the
left
map. The scale at the bottom of each map is the approximated arc length
corresponding to the angle
ʸ
indicated by the abscissa. (
c
) The relationships between
v
ʔt
ƹ (
ʔt
= 2.72 s)
and
ʺ
ʔs
(
ʔs
= 12.5 pixels) in Pp (
top right
), Pm (
top left
), Mp (
bottom right
) and Mm (
bottom left
)
domains. At the right, the percentage of the domains showing a negative correlation between
v
ʔt
ƹ and
ʺ
ʔs
is plotted against
v
i
ƹ
max
which is a measure of the magnitude of the relative protruding activity for
Pp and Mp domains. At the left, the percentage of the domains showing a negative correlation
between
vʔ
t
'
and
ʺ
ʔs
is plotted against
v
j
ƹ
min
which is a measure of the magnitude of the relative
retracting activity for Pm and Mm domains. The
dotted lines
indicate 50% (Adapted with permis-
sion from The Royal Society of Chemistry: [Integrative Biology], copyright (2012))
