Biology Reference
In-Depth Information
SO
3
H
SO
3
H
HO
3
S
SO
3
H
HOOC
Cl
COOH
HO
3
S
SO
3
H
N
+
N
N
+
N
N
+
N
(CH
2
)
5
COOH
(CH
2
)
5
COOH
(CH
2
)
5
COOH
(CH
2
)
5
COOH
HO
3
S(CH
2
)
4
(CH
2
)
4
SO
3
H
Cy7
Cy5.5
NIR-820
l
abs
= 750 nm
l
abs
= 674 nm
= 200,000 M
-
1
cm
-
1
= 190,000 M
-
1
cm
-
1
e
l
abs
= 790 nm
e
l
em
= 694 nm
l
em
= 777 nm
= 184,000 M
-
1
cm
-
1
e
F
f
= not available
F
f
= 0.23
l
em
= 820 nm
Solvent: PBS
Ref. 29
Solvent: PBS
Ref. 30
F
f
= 0.08
Solvent: methanol
Ref. 31
COOH
S
N
+
N
(CH
2
)
4
SO
3
H
N
+
N
+
N
N
(CH
2
)
4
SO
3
H
(CH
2
)
2
COOH
(CH
2
)
2
COOH
HO
3
S(CH
2
)
4
(CH
2
)
4
SO
3
H
ICG
Cypate
CyTE-822
l
abs
= 780 nm
l
abs
= 783 nm
l
abs
= 822 nm
= 224,000 M
-
1
cm
-
1
= 128,000 M
-
1
cm
-
1
= 211,200 M
-
1
cm
-
1
(in H
2
O/MeOH, 75/25)
e
e
e
l
em
= 810 nm
l
em
= 807 nm
l
em
= 847 nm
F
f
= 0.08
F
f
= 0.025
F
f
= 0.15
Solvent: water/20% DMSO
Refs. 34,35
Chart 3.1 Structures and optical properties of representative red and near-IR emitting cyanines.
Solvent: PBS/20% DMSO
Ref. 36
Solvent: PBS
Refs. 32,33
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