Biology Reference
In-Depth Information
A
e
= 38
1.0
Polylysine
Melittin
0.8
0.6
e
= 10
0.4
0.2
0.0
450
500
550
Wavelength (nm)
600
650
B
Melittin
Polylysine
+
N
+
N
+
+
N
+
+
-
N
-
-
-
O
O
O
O
O
+
O
O
O
O
O
O
O
+
P
O
P
O
P
O
+
P
O
+
O
O
O
O
O
O
O
O
O
O
O
O
17 Å
O
O
O
O
8 Å
Figure 2.10 Monitoring the interaction of melittin and polylysine labeled with the 3HC-A
fluorophore with lipid membranes. (A) Fluorescence spectra of labeled melittin and
polylysine bound to model lipid membranes. (B) Schematic presentation of the insertion
of the peptides into the lipid membrane.
66
solvatochromic dyes has already been developed, there is a clear need for
new dyes presenting high solvatochromism, brightness, and photostability.
3HCs are particularly interesting examples of solvatochromic dyes because
of the strong solvent sensitivity of their dual emission generated by ESIPT
Sensitivity of the dyes to the environment polarity and hydration can be
applied for probing the interaction between biomolecules. Being covalently
attached to peptides, they enable monitoring conformation changes of
proteins and their interactions with nucleic acids, proteins, and lipid mem-
branes. Therefore, solvatochromic dyes could become a universal tool for
detecting almost any kind of biomolecular interactions. However, the
further success of the approach will strongly rely on the development of
new improved dyes.
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