Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions - Progress in Molecular Biology and Translational Science - page 50

A

O

H
N

HO

O

O

O

+SL2 (RNA) +PBS (DNA)

B

1.2 1.2

1.0 1.0

0.8 0.8

0.6 0.6

0.4 0.4

0.2 0.2

0.0 0.0

350 400 450

Wavelength (nm)

500 550 600 350 400 450

Wavelength (nm)

500 550 600

Figure 2.8 (A) Schematic structure of a conjugate of the 3HC-B label with the (11 55)

sequence of the NCp7 protein and its complexes with different oligonucleotides, SL2
and PBS, described by NMR. 56,57 (B) Fluorescence spectra of the fluorescently labeled
conjugate (NC-3HC) on addition of SL2 and PBS oligonucleotides. 58 Reproduced from
Ref. 7.

-

studies. 53 A carboxylic acid derivative of 3HC-B was attached to the
N-terminus of the ( 11-55 ) sequence of the nucleocapsid protein (NCp7)
from human immunodeficiency virus (HIV-1) using solid-state peptide
synthesis ( Fig. 2.8 ). This protein plays an important role in HIV-1
through interactions with the viral RNA and its DNA copies, 59

and

notably with cTAR, the complementary DNA sequence of the

from the HIV-1 genome. 60 transactivation response element The

interaction of the labeled NCp7 fragment with cTAR changed