Fluorescent Proteins as Visible In Vivo Sensors - Progress in Molecular Biology and Translational Science - page 387

CHAPTER TEN

Fluorescent Proteins as Visible
In Vivo Sensors

Robert M. Hoffman * , †
* AntiCancer, Inc., San Diego, California, USA
† Department of Surgery, University of California, San Diego, California, USA

Contents
1. Introduction 390

2. Noninvasive Imaging 390

2.1 Noninvasive cellular and subcellular imaging in vivo 391

2.2 Noninvasive cellular and subcellular imaging of cancer cell - stromal cell
interaction 392

2.3 Noninvasive cellular imaging in graft versus host disease 392

3. Lighting Up the Tumor Stroma with Fluorescent Proteins 393

3.1 Imaging the recruitment of cancer-associated fibroblasts by liver-metastatic
colon cancer 393

3.2 Multicolor palette of the TME 394

4. Stroma Cells are Required for Cancer Metastasis 395

5. Fluorescent Tumorgrafts made from Human Cancer Patients 396

5.1 Multicolor palette of fluorescent proteins for lighting up patent tumors
in mouse models 396

5.2 Lighting up metastasis from patient tumors in mouse models 396

5.3 Noninvasive fluorescence imaging of patient tumors in mouse models 396

6. Real-Time Imaging of Trafficking Cancer Cells 397

6.1 Real-time imaging of deforming cancer cells in capillaries 397

6.2 Real-time imaging of trafficking cancer cells in blood vessels 398

6.3 Imaging the trafficking of cancer cells in lymphatic vessels 398

6.4 The role of the intravascular microenvironment in spontaneous metastasis
development 398

6.5 Imaging of nuclear - cytoplasmic dynamics, proliferation, and cell death
of cancer cells in the portal vein area

399

6.6 Imaging the effect of cyclophosphamide on cancer cells quiescence,
proliferation, and death in blood vessels 399

7. Method of Choice for Whole-Body Imaging 400

8. Conclusions 400

References 400