Fluorescent Macromolecular Sensors of Enzymatic Activity for In Vivo Imaging - Progress in Molecular Biology and Translational Science - page 361

Table 9.1 Enzyme-activated NIR-dye-labeled macromolecular imaging probes and their use for optical imaging in vivo

Fluorophore,
mol/mol carrier
macromolecule Animal model

Macromolecule,
type

Target/background
ratio a

Imaging
method

Specificity Reference

PGC b PE c

Cathepsin
B/H/L/S

Cy5.5, 11 Murine breast

adenocarcinoma

12 67

PGC Cathepsin

B/H/L/S

Cy5.5, 11 Murine breast tumor 9.4 (250 pM dye) and

27.5 (2.5 nM dye)

FMT 68

PGC Cathepsin D Cy5.5, 22 Murine hCaD-

expressing tumors

22 PE 69

PGC MMP2 Cy5.5, 12 Murine fibrosarcoma 3 FRI 70

PGC Cathepsin

B/H/L/S

Cy5.5 Murine atherosclerosis

(NO and ApoE
knockouts)

71

PGC Cathepsin

B/H/L/S

Cy5.5 Murine breast tumor 5 FMT 72

PGC Thrombin Cy5.5 Murine thrombosis 2 FRI 73

PGC Cathepsin

B/H/L/S

Cy5.5 Murine dysplastic

intestinal adenomas

2-4.5 FRI 74

PGC Cathepsin

B/H/L/S

Cy5.5 Murine breast cancer 1.5 times higher signal

in invasive versus
noninvasive tumors

FRI 75