Time-Resolved Förster Resonance Energy Transfer-Based Technologies to Investigate G Protein-Coupled Receptor Machinery: High-Throughput Screening Assays and Future Development - Progress in Molecular Biology and Translational Science - page 287

Table 7.1 Summary of TR-FRET-compatible assays for GPCR — cont'd

GPCR signaling
cascade and
downstream events

Biological
measurement

Available TR-FRET assay
(company)

Illustrative
paper Basis

G protein-
independent
pathways

Akt
phosphorylation

Phospho-Akt (Ser473)
(Cisbio Bioassays)
Phospho-Akt (Thr308)
(Cisbio Bioassays)

Sandwich immunoassay: detection of
phosphorylated Akt by production of TR-FRET
signal between an acceptor-labeled anti-Akt
antibody and a donor-labeled anti-phospho-Akt
antibody

Degorce
et al. 12

Specific metabolites Examples: Insulin, cortisol,
cGMP, ADP, etc. (Cisbio
Bioassays)

Competition assay Degorce

et al. 12

Dimerization GPCR Tag-lite (Cisbio

Bioassay)

TR-FRET signal detection between a
donor-labeled SNAP-tagged GPCR A and an
acceptor-labeled SNAP-, CLIP-, or
HALO-tagged GPCR B

Maurel
et al. 26
Doumazane
et al. 27