Fluorescent Sensors of Protein Kinases: From Basics to Biomedical Applications - Progress in Molecular Biology and Translational Science - page 243

Table 6.3 Fluorescent peptide and protein biosensors of protein kinases—cont'd
Protein kinase

Biosensor features Author and year Reference

Akt, PKA, MK2 Mg 2 þ dependent, Sox based,
cell lysates

Shults et al. (2006) 118

Mg 2 þ dependent, Sox based,
multiplexed assay in cell lysates

PKC, PKA, Akt1,
MK2, CDK2,
PIM2

Shults et al. (2005) 119

Mg 2 þ dependent, Sox based,
recognition-domain focused
chemosensors

PKC, Pim2, Akt1,
MK2, PKA, Abl,
Src, IRK

Lukovic et al.
(2008), Gonzalez-
Vera et al. (2010)

120,121

Mg 2 þ dependent, Sox based,
protein-based docking domain
(Sox-PNT)

ERK1, 2 Lukovic et al.

(2009)

122

Mg 2 þ dependent, Sox based,
protein-based docking domain
(Sox-MEF2A)

p38 alpha Stains et al. (2011) 123

CDK9/cyclin T Hybrid biosensor: WW domain
of Pin1 and a Zn 2 þ -DPA
complex

Anai et al. (2007) 124

Src and Abl Lanthanide biosensors:

carbostyril group sensitizer,
Tb 3 þ or Eu 3 þ chelator

Tremblay et al.
(2008)

125

Quenching/unquenching strategies

Src kinase peptide
biosensor

Self-reporting biosensor:
tyrosine quencher/pyrene
group

Wang et al. (2006) 126

Src kinase peptide
biosensor

Self-reporting biosensor:
tyrosine quencher/Cascade
Yellow, Cascade Blue, or
Oregon Green

Wang et al. (2006) 127

Bcr-Abl and Lyn Self-reporting biosensor:
tyrosine quencher/Cascade
Yellow and Oxazine

Wang et al. (2010) 128

PKA Deep quench: pyrene/Rose

Bengal/14-3-3 phosphoserine
binding domain

Sharma et al. (2007) 129

PKA Deep quench: pyrene/

Coumarin probe and Acid

Agnes et al. (2010) 130