Biology Reference
In-Depth Information
CHAPTER SIX
Fluorescent Sensors of Protein
Kinases: FromBasics to Biomedical
Applications
Thi Nhu Ngoc Van, May C. Morris
CRBM-CNRS-UMR 5237, Chemical Biology and Nanotechnology for Therapeutics, Montpellier, France
Contents
1.
Introduction
219
1.1 Protein kinases Structure, function, and mechanism of action
219
1.2 Strategies for probing and studying protein kinases in vitro and in cellulo
222
2. Genetically Encoded Reporters of Protein Kinases
224
2.1 Phosphotyrosine kinase (PTK) biosensors
230
2.2 Biosensors for protein kinase PKA, PKB, PKC, and PKD
232
2.3 Genetically encoded ERK/MAPK biosensors
235
2.4 Cell cycle kinases: ATM, Plk, Aurora, CDK/cyclin biosensors
236
2.5 Other kinase biosensors
238
3. Fluorescent Peptide/Protein Biosensors
241
3.1 Environmentally sensitive kinase biosensors
243
3.2 Metal-ion-induced, chelation-enhanced fluorescence
247
3.3 Biosensors involving quenching - unquenching strategies
250
3.4 Ratiometric strategies
253
3.5 Optimizing peptide biosensors through quenching and caging strategies
254
3.6 Peptide biosensors for cell cycle targets: CDKSENS and CDKACT
256
4. Applications
259
4.1 Fundamental studies of protein kinase localization, function, and regulation
259
4.2 Monitoring protein kinase biomarkers in clinical diagnostics
260
4.3 Drug discovery strategies HTS/HCS assays and postscreening evaluation
261
5. Conclusions and Perspectives
262
5.1 Multicolor imaging Multisensing, multiplex, or multiparameter imaging
263
5.2
In vivo
imaging of protein kinase activities
264
5.3 Delivery, targeting, and activation strategies
264
5.4 Looking toward the future Biomedical imaging for diagnostics
266
Acknowledgments
266
References
266
 
Search WWH ::




Custom Search