From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells - Progress in Molecular Biology and Translational Science - page 165

Table 5.1 Properties of frequently used FPs and their implementation as FRET pair for ratiometric and fluorescence lifetime imaging—cont'd
Example of FRET pair fluorescent proteins

Recommended
donors

Recommended
acceptors

Donor excitation
(nm)

Acceptor emission
(nm)

R 0 c FRET pair Laser References

0.10 Allen and Zhang 41 Cyan-yellow meCFP/

mCerulean

eYFP/mVenus 433-452/

433-445

527/528 Violet 4.92

0.09 Harvey et al. 42 Green-red meGFP mRFP1/mCherry/

tdTomato

488 607/610/581 Argon 4.73

a The colors represent the emission fluorescence of the corresponding fluorescent protein.
b Maximum wavelengths of the excitation and emission spectra.
c R 0 :F¨ rster distances r 0 for FRET pairs of fluorescent proteins. R 0 values are given in nanometers.
References 29-42 .
Adapted from Refs. 30,31 .