From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells - Progress in Molecular Biology and Translational Science - page 164

Table 5.1 Properties of frequently used FPs and their implementation as FRET pair for ratiometric and fluorescence lifetime imaging
Main fluorescent proteins

Excitation
peak (nm) b

Emission
peak (nm) b Color a Protein Organism Brightness Photostability Oligomerization References

Cubitt et al. 32 eCFP Cyan Aequorea victoria 433/445 475/503 þþ þþþ Weak dimer

Zacharias et al. 33 meCFP Cyan Aequorea victoria 433/452 475/505 þþ þþþ Monomer

Rizzo et al. 34 Cerulean Cyan Aequorea victoria 433/445 475/503 þþþ þþ Weak Dimer

Rizzo et al. 34 mCerulean Cyan Aequorea victoria 433/445 475/503 þþþ þþ Monomer

Tsien 29 eGFP Green Aequorea victoria 488 507 þþþ þþþþ Weak dimer

Heim et al. 35 meGFP Green Aequorea victoria 488 507 þþþ þþþþ Monomer

Miyawaki et al. 36 Yellow Aequorea victoria 514

þþþþ þþ

eYFP 527 Weak dimer

Nagai et al. 37 Venus Yellow Aequorea victoria 515 528 þþþþ þ Weak dimer

Nagai et al. 37 mVenus Yellow Aequorea victoria 515 528 þþþþ þ Monomer

Matz et al. 38 DsRed Red Discosoma sp. 558 583 þþþþ þþþþ Tetramer

Campbell et al. 39 mRFP1 Red Discosoma sp. 584 607 þþ þ Monomer

Shaner et al. 40 tdTomato Red Discosoma sp. 554 581 þþþþ þþþ Tandem dimer

Shaner et al. 40 mCherry Red Discosoma sp. 587 610 þþ þþþ Monomer