From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells - Progress in Molecular Biology and Translational Science - page 145

6.5 Targeting FRET biosensors to subcellular compartments 194

6.6 In vitro and in cellulo characterization of new FRET biosensors 194

7. Considerations for KAR Measurements 196

7.1 Controlling acquisition systems 196

7.2 Controlling biological samples 200

7.3 Biosensor dynamic range and reproducibility 203

8. Toward Quantitative Approaches in Biological Processes 204

9. Outlook and Perspective 206

9.1 What about multisensing? 206

9.2 Kinase activity measurements in living animals 206

Acknowledgments 207

References 208

ABBREVIATIONS
A acceptor
AKAR A-kinase activity reporter
Akt protein kinase B
AMDI adaptive Monte Carlo data inflation
ATP adenosine triphosphate
BFP blue fluorescent protein
cAMP cyclic adenosine monophosphate
CCD charge-coupled device
cDNA complementary DNA
CFP cyan fluorescent protein
CKAR C-kinase activity reporter
Cp Circularly permuted
CyPET optimized eCFP for FRET
D donor
DC direct current
DCR dual channel ratio
DNA deoxyribonucleic acid
DsRed Discosoma sp. red fluorescent protein
DVR dual view ratio
eGFP enhanced green fluorescent protein
EKAR extracellular regulated kinase activity reporter
Epac exchange protein activated by cAMP
ERK extracellular regulated kinase
FD frequency domain
FHA forkhead associated
FLIM fluorescence lifetime imaging microscopy
FP fluorescent protein
FRET F¨ rster resonance energy transfer
GFP green fluorescent protein
GTPase hydrolase enzyme that can bind and hydrolyze guanosine triphosphate (GTP)
IRF instrumental response function