Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro - Progress in Molecular Biology and Translational Science - page 138

protein/protein, protein/ligand, and protein/membrane interactions in real
time and in a noninvasive manner. The development of accurate equipment
to measure FRET, single molecule, rapid kinetics, fluorescence anisotropy,
and fluorescence polarization together with the access to new chemistry for
fluorescent probes has opened new routes to identify critical protein confor-
mational changes involved in the regulation of major signaling pathways, as
well as aberrant cell behavior and pathological disorders such as cancer.
Therefore, fluorescence technology constitutes a major piece of the puzzle
for the development of future medicine both at the therapeutic and diagnos-
tic levels.

ACKNOWLEDGMENTS
This work was supported by the Centre National de la Recherche Scientifique (CNRS), the
Agence Nationale de la Recherche (ANR- ANR-06-BLAN-0071), and the Institut
National du Cancer (INCA-BIOSENSIMAG). We thank Dr. May C. Morris (CRBM-
UMR5237-CNRS) for her critical reading of the chapter and all members of our

laboratory for the fruitful discussions.

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