Biology Reference
In-Depth Information
H
2
N
5
¢
3
¢
P
O
O
oligonucleotide
O
-
(CH
2
)
3, 6, 12
B
3
¢
-NH
2
modification
O
P
5
¢
3
¢
NH
2
oligonucleotide
O
O
O
-
HO
C
Base-amino modification
5
¢
oligo
O
5
¢
oligo
5
¢
oligo
O
NH
2
5
¢
oligo
H
O
5
O
NH
2
NH
2
O
O
HN
O
O
HN
HN
O
-
NH
2
O
O
O
O
O
P
HN
H
N
O
-
O
-
O
O
O
P
O
O
P
N
N
O
-
O
O
P
N
O
O
H
H
H
O
O
H
O
H
H
H
H
H
H
H
H
O
O
H
H
O
-
O
O
O
O
H
O
P
O
-
O
O
H
O
-
O
P
O
P
O
-
O
P
3
¢
oligo
3
¢
oligo
3
¢
oligo
3
¢
oligo
D
dR-NH
2
modification (deoxyribose-NH
2
)
5
¢
oligo
O
O
-
O
P
O
O
O
NH
2
H
6
H
H
O
H
O
-
O
P
O
3
¢
oligo
Figure 4.8 Fluorescently labeled oligonucleotide. Oligonucleotide labeling can be performed by adding an amine group (NH
2
) for post-
coupling reactions with an activated dye. Labeling is performed at the 5
0
(A) and 3
0
(B) ends by the introduction of primary anime and variable
spacers on the base with different chemistry and linkers (C) and on the deoxyribose, where the dye is attached to the deoxyribose via a six-
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