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A
NH 2
N
O
O -
S
O
N
N
OOO
N
O
O
O
P
N
PPP
OOO
P
P
N
N
N
N
HO
O
O
O
O
O
O
O
N
O
-
O
O
-
-
-
-
-
+
OH
OH
+
4 HN(CH 3 CH 2 ) 3
3 Na
OH
OH
e -ATP : 1,
N
6-Etheno-adenosine-5 ¢ -triphosphate
ATP- g AmNS : Adenosine-5 ¢ -triphosphate--(sulfo-1-naphthyl)amide
NH 2
NH 2
N
O
O
N
N
O
O
O
P
N
P
P
HO
O
O
N
P
P
N
O
HO
N
O
O
O
O
O
O
N
-
-
O
O
O
+
-
-
-
3 (CH 3 CH 2 ) 3 NH
+
OO
OO
3 (CH 3 CH 2 ) 3 NH
O 2 N
NO 2
O
H
+
NH
CH 3
N
-
-
OO
TNP-ADP : 2 ¢ /3 ¢ -
O
-Trinitrophenyl-adenosine-5 ¢ -diphosphate
Mant-ATP : 2 ¢ /3 ¢ -(
N
-Methyl-anthraniloyl)-adenosine-5'-triphosphate
B
NH 2
dye
N
O
O
O
P
N
P
P
HO
NH
O
N
O
N
O - - -
3 (CH 3 CH 2 ) 3 NH
O
HN
O
O
+
O
O
NH 2
O
O
O
O
P
N
H
N
HN
P
P
O
O
O
N
O
N
O
O
O
O -
NH
- -
3 (CH 3 CH 2 ) 3 NH
+
OH
OH
dye
dye
dye
NH
HN
O
NH
N
NH
N
O
O
O
P
N
HN
O
O
P
P
N
N
NH 2
N
HO
O
O
O
N
O
O
O
O
-
-
-
O
P
O
OH
O
+
-
OH
OH
3 (CH 3 CH 2 ) 3 NH
+
(CH 3 CH 2 ) 3 NH
Figure 4.6 Fluorescently labeled nucleotide analog. (A) Most common dyes used on
fluorescently labeled nucleotides on the ribose, the base, and the phosphate moieties.
(B) Amino-hexyl-modified nucleotides for custom-made labeling with a large panel of
dyes.
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