CELL-PENETRATING PEPTIDE FUSION PROTEINS - Fusion Protein Technologies for Biopharmaceuticals: Applications and Challenges - page 384

TABLE 26.1 Selection of Proteins Delivered by CPPs

Protein CPP Application References

Astrocytic phosphoprotein
PEA-15 a

Tat Prevention of apoptosis [17]

Avidin Penetratin, pVEC, Tat,

Transportan

Protein transduction efficiency of four cell-
penetrating peptides

[18]

B-cell lymphoma-extra large
(Bcl-xL) a

Tat Protection against ischemic brain injury and neuronal

apoptosis

[19]

Calcineurin a

Tat Transduction of TAT fusion proteins into osteoclasts

and osteoblasts

[20]

Caspase-3 a

Tat Targeted apoptosis initiation [21]

Cell division control protein 42
(CDC42) a

Tat Rho protein transduction, osteoblast effect [22-24]

Cre recombinase a MTS, Tat penetratin, Manipulation of gene structure and function [25]

Cu, Zn superoxide dismutase
(SOD) a

Tat Transduction of Cu, Zn-superoxide dismutase into

human chondrocytes

[26]

Cyclin-dependent kinase 2
(Cdk2) a

Tat Retinoblastoma tumor suppressor protein regulation [27,28]

Domain III of Pseudomonas
exotoxin A (PE) b

Tat Protein delivery [29]

Double-stranded RNA-binding
domain (DRBD) a

Tat Enhancement of CPP-mediated RNA delivery [30]

Transcription factor (E2F) a Tat Apoptosis activation [31]

Enhanced green fluorescent
protein(eGFP) a

R8, Tat, VP22 Peptide-mediated therapy for pancreatic cancer.

Intracellular delivery to muscle cells. Cellular
uptake of histidine-tagged proteins

[32-34]

Exonuclease III (ExoIII) a

Tat Transduction and targeted delivery into mitochondria

of breast cancer cells

[12]

Flp recombinase a VP22 Intracellular delivery of functional Flp recombinase [35]

Forkhead box P3 (Foxp3) a

HHph-1 Therapeutic effect in autoimmune and allergic

disease models

[16]

Fragment antigen-binding
(antibody fragment) (FAB) b,c

REV, Tat Enhancing uptake of FAB fragments [36,37]

Transcription factor (GATA4) a VP22 Therapeutic effect on ischemic cardiomyopathy [38]

Green fluorescent protein(GFP) a

POD, R9, Tat,
Transportan, VP22

Primary brain cell delivery is dependent on
glycosaminoglycan expression. POD mediates
delivery of recombinant proteins to retina, cornea,
and skin. Fluorescent protein delivery via
arginine-rich peptides to plant cells. Cellular
translocation of proteins by transportan

[39-43]

Heat shock protein 70 (Hsp-70) a Tat Preventing neuronal cell death in in vitro/vivo

models of Parkinson's disease. Neuroprotection of
neuronal precursor cells after focal cerebral
ischemia in mice

[44,45]

Hemagglutinin a Tat Transduction of TAT fusion proteins into osteoclasts

and osteoblasts

[20]

Herpes simplex virus type 1
thymidine kinase (HSV1-TK) a

Tat, VP22 Intracellular delivery of proteins [46]

Horse radish peroxidase (HRP) b Tat Protein delivery [29]

Inhibitor of Kappa B (I k B a ) a Tat Diminished infiltration of leukocytes into the site of

inflammation

[47]

Insulin K9, R9, Tat Increased pulmonary absorption of insulin [48]

Nuclear receptor corepressor
fragments (N-CoR) a

Tat Unblockage of differentiation in leukemia cells [49]

Ovalbumin a TLM Enhancement of protein vaccination [50]

p16 a

Tat Cancer therapy [51]

p27 a Tat Manipulation of cell migration [52]