Biomedical Engineering Reference
In-Depth Information
TABLE 14.8
Examples of DNA Biosensors
Immobilized
DNA
Signal
Transducers
Immobilization Method
of DNA
Substrates
Type
Ref.
Au disk electrode
The thiol-tagged probe DNA
and 6-mercapto-1-hexanol
were immobilized on the
surface of electrode through
thiol-Au binding
Electrochemical
DNA biosensor
130
ssDNA
Complementary
target
oligonucleotide
Au substrates
DNA was immobilized by
forming covalent amide bonds
between carboxyl groups at
the CNTs and amino groups at
the ends of the DNA
oligonucleotides
Electrochemical
DNA biosensor
131
Ag-plated
surface
DNA was immobilized by the
active coating of bovine serum
albumin (BSA)
Acoustic wave
DNA biosensor
133
Au-covered
quartz surface
DNA was immobilized on the
gold quartz surface in NaCl
solution
Quartz crystal
microbalance
(QCM) DNA
biosensor
134
Biotinylated
oligonucleotide
(ssDNA)
Complementary
target
oligonucleotide
Resonant mirror
Biotinylated oligonucleotide
was immobilized on the sensor
surface by streptavidin
Optical DNA
biosensor
132
dsDNA
Mitoxantrone
Glassy carbon
electrode
Electrochemical
DNA biosensor
135
Toxic aromatic
amines
Screen-printed
electrodes
Electrodes were immersed in a
stirred acetate buffer solution
containing dsDNA for 120 s at
a potential of
+
0.5 V versus
SCE
Electrochemical
DNA biosensor
136
Benzo[a]pyrene
(BaP)
Carbon paste
electrode
dsDNA was immobilized on the
electrode by applying a
potential of
+
0.50 V for 5 min
in 10 ppm dsDNA with
200 rpm stirring
Electrochemical
DNA biosensor
137
Organic analytes
and metal
Flow cell fl uidics
dsDNA was immobilized on a
silica surface through avidin-
biotin coupling
Waveguide DNA
biosensor
138
Denatured
DNA
Autoantibodies
in blood
serum
Stationary
mercury fi lm
covered silver
electrode
Denatured DNA was
immobilized on cellulose
nitrate fi lm
Amperometric
139
14.2.1 C
ARBON
N
ANOTUBES
Carbon nanotubes (CNTs) show intriguing performance when used as intermedia materials for
biosensors. CNTs, which were discovered by Iijima in 1991 [151] and represent a new kind of carbon
material. They are found in two distinct types of structures, namely, single-wall CNTs (SWCNTs) and
multiwall CNTs (MWCNTs). SWCNTs comprise a cylindrical graphite sheet of nanoscale diameter
