Biomedical Engineering Reference
In-Depth Information
(A)
Substrate
Upper cup
Substrate
solution
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Product
solution
Lower cup
Products
(B)
GA: Glucoamylase
GOD: Glocose oxidase
POD: Peroxidase
Glucono-
δ
-lactone
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FIGURE 12.20
(A) Structure of a multienzyme reactor composed of GA and GOD. (B) Sequential reaction
catalyzed by the multienzyme reactor. In order to monitor the reaction, DA67 oxidation by POD in the fi ltrate
is coupled with the sequential reaction in the fi lm.
procedure excludes the use of specialized apparatus, making the method inexpensive. The assembly
process is conducted at enzyme-friendly conditions, and therefore, denaturation of a protein can be
minimized. An increase in stability of the enzyme immobilized in the assembled fi lm was also real-
ized as one of the advantages in reactor functions. In addition, the freedom of the selection of solid
supports in fi lm preparation activates our imagination to design protein-based nanodevices.
Since surfaces of lipid membranes act as a good medium to accommodate both biological
components and artifi cial functional molecules, connection of different kinds of functions at the
membrane surface is a promising approach to construct novel types of supramolecular devices.
Hybridization of artifi cially designed functions to naturally occurring enzymatic catalyses would
