Electrohydrodynamic Processing of Micro- and Nanometer Biological Materials - Biomaterials Fabrication and Processing - page 309

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manifold

Main manifold

Transfer
rod

ESI

X

Y

Z

Gate
valve

Surface
positioning stage

(10 − 6 Torr)

Linear ion
trap

(10 − 3 Torr)

(10 − 5 Torr)

10 − 3 Torr)

(10 − 4 to

FIGURE 11.33 A schematic of soft-landing instrument including electrospraying setup. (Reprinted from
Blake, T.A. et al., Anal. Chem. , 76, 6293, 2004. © American Chemical Society. With permission.)

(a) (b)

Mixture of
I, L, T, C

I L

C T

(d)

(c) +9

+1

+4

L

I

+8

+11

+5 +3

(e) (f)

+8

C

T

+9

+8

+7

FIGURE 11.34 An array of different spots prepared using electrosprayed ion soft landing from a protein
mixture: (a) spectrum of the four proteins insulin (I), lysozyme (L), trypsin (T), and cytochrome c (C); (b)
deposition of protein array; (c)-(f) spectrum of individual protein spot. (Reprinted from Blake, T.A. et al.,
Anal. Chem. , 76, 6293, 2004. © American Chemical Society. With permission.)