Biomedical Engineering Reference
In-Depth Information
Glass
condensor
encasi
ng syringe
with po
lymer
A
Collection screen
B
Syringe pump
Power supply
Circulating hot
water
C
(a)
A
B
(b)
FIGURE 5.9
(a) Setup of electrospinning of polymeric materials from the melt. Basic system consists of a
syringe pump, a high-voltage power supply, and a heating circulator to facilitate melting and maintenance of
high temperature for the polymer (A and B). The collected melt electrospun fi bers are shown in (C). Insert in
C shows a higher magnifi cation of the melt-spun fi bers. (b) Direct
in vitro
melt electrospinning of PCL-PEG
block copolymer onto bone marrow cells pre-seeded on PCL/Col nanofi ber mesh. (A) Live-death assay using
FDA-PI revealed relatively good viability of the cells after 1 week of culturing post-spinning. (B) Electron
micrograph after 1 week culturing post-spinning showing a cell attaching to both PCL/Col nanofi ber substrate
and melt-spun PCL-PEG.
