Biology Reference
In-Depth Information
Fig. 3. Block diagram of the controlled environment vitrification system used to
prepare vitreous-ice samples for transmission electron cryomicroscopy.
7.
Transfer the holder quickly into the microscope specimen airlock to prevent
warming up and to minimize frosting from atmospheric water vapor. Once the
holder is in the high vacuum of the microscope column, leave it for 15 min to
allow the temperature to stabilize and for the vacuum to remove any frosting
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