Semipermeabilized Cells to Study Procollagen Assembly - Methods in Molecular Biology: Extracellular Matrix Protocols - page 3

Fig. 1. Procollagen folding occurs within semipermeablized cells: RNA coding for
procollagen was translated in the presence of semipermeabilized cells ( see text for
details).

5. HEPES buffer: 50 m M KOAc, 50 m M HEPES, pH 7.2.

6. 50 mg/mL soybean trypsin inhibitor, in sterile water stored at -20 ° C (Sigma, St.

Louis, MO).

7. 40 mg/mL digitonin in DMSO, stored at -20 ° C (Calbiochem, La Jolla, CA).

8. 0.4% Trypan blue solution.

9. 0.1 M CaCl 2 (stored at -20 ° C).