Fluorescence Assays to Study Cell Adhesion and Migration In Vitro - Methods in Molecular Biology: Extracellular Matrix Protocols - page 310

results. This because, in this case, the majority of the transmigrated cells remains bound
to the underside of the membrane. Thus, fluorescence measurement from either the
top or bottom side of the membrane would unavoidably cause detection of the fluores-
cence signals emitted from both cell populations.