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8.
At this stage, if more convenient, fixation in gel fixing solution 1 can be omitted
and the gel can be placed straight into gel fixing solution 2 for overnight fixation.
Acknowledgments
The author would like to thank Dr. Lynda F. Bonewald for critical review of
this manuscript and Frances J. Ramirez for expert secretarial help. Financial
support from the National Institutes for Health and the National Osteoporosis
Foundation is also gratefully acknowledged.
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