Biology Reference
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Fig. 2. Typical AFM approach and retract cycles for glyconectin 1 to glyconectin 1
interactions. The
x
-axis shows the vertical movement of the cantilever and the
y
-axis
its bending and, thus, the force acting on it.
A, B, C
and
D
represent typical glyconectin
1 to glyconectin 1 interactions, whereas
E
is an example of the interaction between
two gold surfaces not covered with selfassembled monolayers (11-thio-undodecanol).
12.
Carbonyldiimidazole. Keep sealed in dry place at room temperature.
13.
Crosslinking buffers. Choice depending of the nature of the molecule. Recom-
mended is 0.1-0.5
M
NaCl, 20 m
M
HEPES buffer pH 7.4. (
See
Note 6
.)
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