Biology Reference
In-Depth Information
Acknowledgments
We thank Dr. Rasmus Schröder for helping us to collect energy-filtered TEM
images of collagen fibrils in vitreous ice, using a Zeiss 912
instrument. This
work was supported by the Wellcome Trust (019512). Karl E. Kadler is a
recipient of a Senior Research Fellowship from the Wellcome Trust.
References
1. Chapman, J. A., Tzaphlidou, M., Meek, K. M., and Kadler, K. E. (1990) The
collagen fibril—a model system for studying the staining and fixation of a pro-
tein. Electron Microsc. Rev . 3, 143-182.
2. Holmes, D. F., Watson, R. B., Steinmann, B., and Kadler, K. E. (1993) Ehlers
Danlos syndrome type VIIB. Morphology of type I collagen fibrils is determined
by the conformation of the N-propeptide. J. Biol. Chem. 268, 15,758-15,765.
3. Holmes, D. F., Chapman, J. A., Prockop, D. J., and Kadler, K. E. (1992) Growing
tips of type I collagen fibrils formed in vitro are near-paraboloidal in shape,
implying a reciprocal relationship between accretion and diameter. Proc. Natl.
Acad. Sci. USA 89, 9855-9859.
4. Dubochet, J., Adrian, M., Chang, J. J., Homo, J. C., Lepault, J., McDowall, A. W.,
and Schultz, P. (1988) Cryo-electron microscopy of vitrified specimens. Quart.
Rev. Biophys. 21, 129-228.
5. Raspanti, M., Alessandrini, A., Gobbi, P., and Ruggeri, A. (1996) Collagen fibril
surface: TMAFM, FEG-SEM and freeze-etching observations. Microsc. Res.
Techn. 35, 87-93.
6. Heuser, J. (1989) Procedure for 3-D visualisation of molecules on mica via the
quick-freeze, deep-etch technique. J. Electron Microsc. Techn. 13 , 244-263.
7. Bellare, J. R., Davis, H. T., Scriven, L. E., and Talmon, Y. (1988) Controlled
environmental vitrification system: an improved sample preparation technique. J.
Electron Microsc. Techn. 10, 87-111.
8. Holmes, D. F., Capaldi, M. J., and Chapman, J. A. (1986) Reconstitution of col-
lagen fibrils in vitro ; the assembly process depends on the initiating procedure.
Int. J. Biol. Macromol. 8, 161-166.
9. Sherratt, M., Graham, H. K., Kielty, C. M., and Holmes, D. F. (1999) ECM mac-
romolecules: rotary shadowing and scanning transmission electron microscopy.
Methods in Molecular Biology , Extracellular Matrix Protocols (Streuli, C. and
Grant, M., ed.), Humana Press, Totowa, NJ.
10. Schröder, R. R., Hofmann, W., and Menetret, J. F. (1990) Zero-loss energy filter-
ing as improved imaging mode in cryoelectron microscopy of frozen hydrated
specimens . J. Struct. Biol . 105, 28-34.
11. Langmore, J. P. and Smith, M. F. (1992) Quantitative energy-filtered electron
microscopy of biological molecules in ice. Ultramicroscopy 46, 349-373.
Search WWH ::




Custom Search