The Structural and Functional Uniqueness of the glmS Ribozyme - Progress in Molecular Biology and Translational Science - page 180

A

B C

Figure 5.2 Importance of coenzyme functional groups to glmS self-cleavage. (A) A sur-
face view of GlcN6P in the active site of the glmS ribozyme (PDB ID: 2NZ4 rendered using
Chimera). 13,22 - 24 (B) Functional group modifications of GlcN6P that diminish activity to
1% are highlighted in green. (C) Ring opening and functional group modifications of
GlcN6P that diminish activity to < 1% or entirely are highlighted in red.

contribute to ligand binding or RNA self-cleavage. 18 Finally, the glmS
ribozyme from B. anthracis binds with selectivity to the a -anomer of GlcN6P
and binding is pH-dependent. 36 These results and previous work 6,18,19,36
indicate the importance of stereospecificity and protonation state in ligand
binding to the RNA.