Biology Reference
In-Depth Information
A
B
C
G
J1/2
C
U
U
C
G
C
3
10
30
A
A
A
A
A
U
G
G
A
A
G
G
G
U
C
U
U
U
C
C
C
A
A
C
C
U
G
C
80
C
A
G
U
C
G
G
G
U
C
G
G
C
U
U
G
G
C
U
C
U
A
C
P2
20
A
P1
G
20
G
40
1
P3
U
G
G
C
40
U
5
J1/2
HO
G
G
C
30
G
G
A
CG
U
C
C
P1.1
A
A
C
C
J4/2
G
A
C
U
C
C
U
A
3
A
U
U
U
A
C
G
U
U
G
U
70
10
G
A
G
G
G
180
A
A
P4
50
A
A
A
U
U
C
C
G
G
C
A
G
G
C
C
50
C
G
G
C
P2
U
A
P1
60
C
G
U
A
A
G
170
C
G
G
A
A
A
P3
U
A
G
C
5
HO
70
60
G
C
L4
P1.1
G
C
A
C
G
U
C
G
A
A
U
C
80
G
C
U
U
A
J4/2
J1.1/4
C
166
AG
G
C
10
-
1
A
U
P4
Total ribozyme
Uncleaved ribozyme
U
A
G
C
C
10
-
2
G
160
G
U
C
90
A
10
-
3
U A
AU
C
G
10
-
4
U
G
AU
10
-
5
57 nt
Larva
Pupa
Female
Male
Figure 4.7 Secondary structures for ribozymes from the (A) drz-Agam-1 and (B) drz-
Agam-2 families, corresponding to the drz-Agam-1-1 and drz-Agam-2-1 ribozymes,
respectively. (C) In vivo expression data obtained by qPCR for the drz-Agam-1-1
ribozyme. Expression for other drz-Agam ribozymes follows a similar pattern.
overall folding of the motif, as such sequences exhibit rapid cleavage with
low sensitivity to Mg
2
รพ
and temperature.
3
The
in vivo
expression of the drz-Agam ribozymes was confirmed
through 5
0
RACE experiments and RT-qPCR. Expression was found to
vary between sexes and life stages of the insect, with the highest expression
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