Biology Reference
In-Depth Information
A
G
Md
Bt
J1/2
3
10
A
C
A
U
C
A
Hs, Dn
Other
mammals
30
G
A
A
A
C
U
U
C
A
C
G
C
G
P2
G
C
P1
A
C
Cf
G
C
G
C
G
C
Hs SNP
Bt, Rn
Cf
Dn
U
G
C
A
U
C
G
U
G
G
U
C
U
5
P3
Cf
Bt
L3
G
C
A
U
HO
U
U
G
C
A
C
G
U
C
G
C
A
P1.1
A
J4/2
60
U
U
U
A
20
G
C
A
U
Cf
Dn
C
G
U
57
40
A
G
C
P4
A
U
U
A
A
G
C
50
U
A
A
U
C
Mm
Dn
G
G
A
G
Md
U
A
B
hCPEB3 protein
Q
RRM-1
RRM-2
Znf
mRNA
346 aa
698 aa
Rz
hCPEB3
gene
Chr 10:
94,000,118
93,808,399
C
Consensus
AGGGGGCC AC AGC AGA AGCGT T C ACGT C - GCGGCCCCT GT C AGAT T CT GGT GA AT CT GCGA AT T - CT GCT G
Figure 4.5 (A) Secondary structure of the human CPEB3 ribozyme. SNPs and mutations
of the ribozyme sequence in other mammals are indicated in boxes. Bt, cow; Cf, dog; Dn,
armadillo; Hs, human; Md, opossum; Mm, mouse. (B) Genomic location of the human
CPEB3 ribozyme. (C) Consensus alignment of the CPEB3 ribozyme found in various mam-
malian species.
Several studies have indicated that the catalytic rate of the HDV ribozyme
can be modified upon the inclusion of 5
0
and 3
0
flanking sequences.
50,55,56,59,60
For instance, destabilization of the formation of alternate P1 and P3 helices by
mutation of G11 to C and removal of the terminal U from the L3 region of
genomic HDV enhances the catalytic rate by over three orders of magni-
tude.
60
The catalytic rates of the
CPEB3
ribozymes appear to be affected
in a similar manner. Recently, the human version of the ribozyme was found
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