Chemistry Reference
In-Depth Information
8
1
POPC
EYPC
0.8
6
0.6
4
0.4
2
0.2
0
0
5 7 10
14 14
912 1616 129
10 7
5
5
710
1616
1075
(a)
(b)
POPC
EYPC
4
3
2
2
×
×
×
×
1
1
0
T
5 7 10 14
14 10 7 5
T
T
5
9
12
16 16
12
9
5
T
(d)
(c)
912 16 16 12 9
POPC
DOPC
2
64
1.5
68
1
0.5
72
×
×
0
T
5
7
10
14
14
10
7
5
T
5
7
10
16 16
10
12
7
5
(e)
(f)
9
12
16 16
12
9
912
9
FIGURE 10.16
Proi les of different properties across PC (○) and PC/Chol (
●
) membranes. The approximate
locations of nitroxide moieties of spin labels are indicated by arrows. (a) Order parameter in POPC mem-
branes with and without 50 mol% cholesterol at 25°C. (b) Bimolecular collision rate for a nitroxide moiety
at the C16 position of the stearic acid alkyl chain with other SASLs in EYPC membranes with and without
10 mol% lutein at 27°C. (c) Oxygen transport parameter in POPC membranes with and without 50 mol%
cholesterol at 25°C. (d) Relative no diffusion-concentration product in EYPC membranes with and without
30 mol% cholesterol at 20°C. (Adapted from Subczynski, W.K. et al.,
Free Radic. Res
., 24, 343, 1996. With
permission.) (e) Hydrophobicity proi les (2
A
Z
) in POPC membranes with and without 50 mol% cholesterol
(results obtained at −165°C). Upward changes indicate increases in hydrophobicity. To relate hydrophobicity
as observed by
A
Z
at a selected depth in the membrane to hydrophobicity (or ε) of bulk organic solvent, see
Figure 10.5c. (f) Penetration of Fe(CN)
−3
(
P
(
x
), dei ned by Equation 10.8) into the DOPC membranes with and
without 30 mol% cholesterol at 25°C from the buffer containing 50 mM K
3
Fe(CN)
6
. (From Widomska, J. et al.,
Biochim. Biophys. Acta
, 1768, 1454, 2007. With permission; Subczynski, W.K. et al.,
Biochemistry
, 33, 7670,
1994. With permission; Subczynski, W.K. et al.,
Biochemistry
, 42, 3939, 2003. With permission; Yin, J.J. and
Subczynski, W.K.,
Biophys. J
., 71, 832, 1996. With permission.)
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