Application of Resonance Raman Spectroscopy to the Detection of Carotenoids In Vivo - Carotenoids: Physical, Chemical, and Biological Functions and Properties - page 93

Video camera

LCD

BS Eye

CCD
camera

Shutter Eyepiece

Filter

L3

White light
source

NF L4

LED

BS

Fiber
bundle

VHTG

Trigger

Synchronizing
electronics

Shutter

F L2 L1

Ar + laser

M Fiber

(a)

(b)

14000

Calibration

12000

10000

8000

6000

4000

2000

0

0.0 0.2 0.4 0.6

Optical density at 488 nm

0.8 1.0

(c)

FIGURE 6.5 (a) Schematics of fundus-camera-interfaced RRS instrument for measurement of integral MP
concentrations in human clinical studies; (b) computer monitor display showing raw Raman spectrum obtained
after single measurement (left panel) and processed, scaled spectrum obtained after subtraction of l uorescence
background (right panel); and (c) calibration curve for RRS response of tissue phantom for nine lutein and
zeaxanthin concentrations. (From Ermakov, I.V. et al., J. Biomed. Opt ., 9(1), 139, 2004. With permission.)