Biomedical Engineering Reference
In-Depth Information
roughly equal (Fig 7b); however, after 14 days the ALP activity of BMSCs in the 5.0%Silk-
MBG scaffolds was greater than that of the other two scaffold types (Fig. 7b). The
osteoblastic differentiation was further assessed by the mRNA expression of ALP and OCN
using RT-qPCR method. There was an upregulation of the osteogenic marker genes of OCN
and ALP after the modification of MBG scaffolds by silk (Fig. 8).
Fig. 6. The morphology of bone marrow stromal cells on the silk-modified and unmodified
MBG scaffolds after 1 and 7 days of culture [19].
From the results above, it is indicated that that silk modification of MBG scaffolds had a
positive effect on the attachment, proliferation and differentiation of BMSCs [19]. Generally,
the ionic environment and material surface are two main factors which influence the
interaction of cells and biomaterials [7,26-29]. In this study, silk modification did not create
any significant effect on either ionic release or weight loss of MBG scaffolds. It has been
speculated that the ionic environment and pH value of culture media may not be the most
important factors affecting the cell response. Therefore, there is a reasonable hypothesis that
