Biomedical Engineering Reference
In-Depth Information
Fig. 4. Cells Viability (MTT). The graph shows cell viability when cultivated on scaffolds for
1 week.
B
A
Fig. 5. Scanning electronic microcopy. (A) and (B) Cells attached to scaffolds.
3.3
In vivo
assay
After 3 weeks of culture in chondrogenic medium in respective scaffolds, we grafted those
constructs (association of scaffolds and cells) subcutaneous onto the dorsal region of Lewis
rats. Those rats were sacrificed with anesthestic overdose on the 3
rd
week after implantation.
These samples were analyzed by immunofluorescence and we verified that collagen type II
was present in both samples. This result showed that once cells differentiated in the scaffold
they do not dedifferentiate. The cells were able to maintained the differentiated phenotype
inside the scaffolds (Fig. 6).
