Biomedical Engineering Reference
In-Depth Information
CD54, MSC markers and CD45 a HSC marker. The goal of this experiment was to observe
wether the cellular phenotype changed during the 9 weeks in chondrogenic medium. It is
important to note that GFP cells are able to fluoresce without secondary antibody. Therefore
anti-GFP were not used , only anti-CDs antibodies and secondary antibodies conjugated to
PE to not GFP used. We observed that when cells were cultivated in chondrogenic medium,
they were phenotypical alterated. Differentiated cells were positive for CD73 and Collagen
II (red, Fig. 2) and they were negative for CD90, CD45, CD54 and osteocalcine (Fig. 2).
Fig. 2. Cells differentiated with chondrogenic medium for 9 weeks. CD45, CD54, CD73,
CD90, Collagen II and osteocalcine. Cells were GFP labeled.
