Mesenchymal Stem Cells from Extra-Embryonic Tissues for Tissue Engineering –Regeneration of the Peripheral Nerve - Advances in Biomaterials Science and Biomedical Applications - page 470

Figure 1. Cross section of an umbilical cord transported immersed in DPBS at the refrigerated temperature of 4-6°C.
Samples were stained with haematoxylin and eosin (HE). Magnification: 10X.
Figure 2. Cross section of an umbilical cord transported immersed in DPBS at the refrigerated temperature of 4-6°C.
Samples were stained with haematoxylin and eosin (HE). The UCT integrity was quality evaluated through the follow‐
ing parameters: i) detachment of vessels and retraction of vascular structures; ii) loss of detail and integrity of the en‐
dothelium; iii) connective tissue degradation; iv) autolysis of fat (impossible to assess, due to histological technique);
and v) loss of detail and integrity of the mesothelium. Magnification: 40X.
As a matter of fact, the UC immersed for 168 hours in DPBS and HBSS at refrigerated tem‐
perature presented integrity of the histological structure comparable to a UC collected and
processed for histological analysis immediately after birth (Figure 3). With DPBS, a slight re‐
traction of the vessels was noted, which is advantageous since the vessels are stripped and
discarded before cryopreservation of the UCT. It was concluded that the transport of the UC