Biomedical Engineering Reference
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Figure 6. SDS-PAGE of purified fractions from AutoBT powder fabricated from a dried tooth in 25 ºC (A) and from
wisdom tooth in fresh state (B).
3. Osteoconduction of AutoBT
The analytic results showed that AutoBT consisted of low-crystalline hydroxyapatite (HA) and
possibly other calcium phosphate minerals (ß-tricalcium phosphate (ß-TCP), ACP, and OCP),
similar to the minerals of human bone tissues. Note, however, that the level of HA crystalli‐
zation and the amount of HA differed greatly depending on the area of the tooth. The XRD
pattern was much stronger in the crown portion with enamel than in the root portion (Figure
7). Likewise, the dental crown portion consisted of high-crystalline calcium phosphate
minerals (mainly HA) with higher Ca/P ratio, whereas the root portion was mainly made up
of low-crystalline calcium phosphates with relatively low Ca/P ratio [3, 23]. Kim, et al [52]
performed the study to evaluate the surface structures and physicochemical characteristics of
a novel autogenous tooth bone graft material currently in clinical use. The material's surface
structure was compared with a variety of other bone graft materials via scanning electron
microscope (SEM). The crystalline structure of the autogenous tooth bone graft material from
the crown (AutoBT crown) and root (AutoBT root), xenograft (BioOss), alloplastic material
(MBCP), allograft (ICB), and autogenous mandibular cortical bone were compared using x-
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