Biomaterials and Stem Cell Therapies for Injuries Associated to Skeletal Muscular Tissues - Advances in Biomaterials Science and Biomedical Applications - page 353

Figure 7. Double imunofluorescence staining for laminin and CD31.

Figure 8. Pax7 + SC counterstained with DAPI.

5.5. Electron microscopy

Electron microscopic (EM) examination of the glutaraldehyde-fixed portion of the biopsy is
performed when the light microscopic studies are inconclusive. Thus, it is reserved for se‐
lected circumstances in which the pathologist determines that EM has the potential of con‐
tributing significantly to determining a specific diagnosis. A specimen placed in
glutaraldehyde must be small, approximately 1-2 mm in width and depth, allowing the
complete tissue penetration by this fixative. Glutaraldehyde makes tissue brittle and inter‐
feres with immunohistochemical studies, so it is not appropriate for the paraffin specimen.
With EM, other muscle cell parameters can be analyzed in detail: the myofibril architecture;