Third party AFM software - Atomic Force Microscopy - page 199

Table C1. Some third party software for processing and analysis of AFM images.

url

Software Package

Gwyddion http://www.gwyddion.net/

SPIP http://www.imagemet.com/

WSxM http://www.nanotec.es/

FemtoScan Online http://www.nanoscopy.net/en/Femtoscan-D.php

PUNIAS http://site.voila.fr/punias

Image SXM http://www.liv.ac.uk/ Esdb/ImageSXM/

ImageJ http://rsb.info.nih.gov/ij/

WSxM - Windows Scanning x Microscope [738]. This is commercial but free software
that enables control of the AFM instruments sold by the company that developed it, but
which is also a processing and analysis software package. It opens many image file
formats and also some force-curve data, and it is updated regularly. All the procedures
described in Chapter 5 can be carried out with WSxM.

FemtoScan Online. Commercial software from an instrument manufacturer, but which is
also a processing and analysis software package that loads several other image file
formats. A 30-day trial version is available for free download.

PUNIAS (Protein Unfolding and Nanoindentation Analysis Software). This free software
is dedicated to analysis of force curves, and force-curve map files. It implements several of
the common algorithms used for analysis of force spectroscopy and nanoindentation data.
It is able to load force curve data from several of the most popular file formats. It is
updated fairly regularly.

Image SXM and ImageJ. Both of these programs are versions of NIH Image , a public
domain image processing and analysis program for the Macintosh. Image SXM is a Mac
OS-only version of NIH Image that has been extended to handle the loading, display and
analysis of AFM images in several file formats. Image J is a cross-platform image analysis
program, again based on NIH Image . Packages are available for Mac OS, Linux and
Windows. It is not specifically designed for SPM images, but there are plugins available to
load a few file formats. These programs are rather limited in their applicability to AFM
files, as they are not by default designed to work with three-dimensional topographical
data, but have many more 'classical' image analysis procedures, and so can be useful
under some circumstances.

A list of urls linking to all the software described here is reproduced in Table C1. An
updated listing is available at http://afmhelp.com.