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A
Mouse E10.5 forebrain ( Mus musculus )
max
Hoxd13
Hoxb13
0
max
Hoxd4
Hoxb4
0
max
H3K27me3
H3K27me3
0
Hoxd13
Hoxd9
Hoxd4
Hoxd1
Hoxb13
Hoxb9
Hoxb4
Hoxb1
50 kb
B
Mouse E10.5 anterior trunk ( Mus musculus )
max
Hoxd13
Hoxb13
0
max
Hoxd4
Hoxb4
0
max
H3K4me3
H3K4me3
ma 0
H3K27me3
H3K27me3
0
Hoxd13
Hoxd9
Hoxd4
Hoxd1
Hoxb13
Hoxb9
Hoxb4
Hoxb1
Inactive
compartment
Active
compartment
Inactive
compartment
Active
compartment
Figure 4.7 3D organization of the murine Hox clusters during spatial collinearity.
(A) Local compartmentalization of inactive Hox clusters in the E10.5 mouse brain. Quan-
titative 4C-seq (Circular Chromosome Conformation Capture) signal and distribution of
the H3K27me3 mark are shown. The HoxD cluster is on the left and HoxB on the right.
The viewpoints Hoxd13, Hoxd4, Hoxb13, and Hoxb4 are indicated. Local 3D compart-
mentalization of the inactive HoxD and HoxB clusters is schematized below. (B) Bimodal
local compartmentalization of Hox clusters along the E10.5 mouse embryonic AP axis.
Quantitative 4C-seq signals and the distribution of the H3K4me3 and H3K27me3 marks
are shown. Hox genes from group 1
8 are active at this body level, in agreement with
spatial collinearity. The same viewpoints as in (A) are shown. Local 3D compartmental-
ization of the HoxD and HoxB clusters along the AP axis is schematized below. Panels
(A) and (B) data from Noordermeer et al. (2011) .
-
shows a similar organization, suggesting that this bimodal structure is
maintained along the AP axis during embryonic development ( Min, Lee,
&Kim, 2012 ). The size of these local compartments varies along the AP axis.
In the caudal part of the embryo, where gradually more Hox genes are tran-
scribed, the active 3D compartment extends further toward the 5 0 end of the
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