LABEL-FREE MONITORING OF CELL SIGNALLING PROCESSES THROUGH AFM-BASED FORCE MEASUREMENTS - Life at the Nanoscale: Atomic Force Microscopy of Live Cells - page 359

(a)
(b)
(c)
(d)
Figure 17.6. Identiication of the signalling pathways involved in the mechanical
response induced by the activation of the AT 1 receptor. (a) Schematization of the
two independent signalling pathways known to be involved in contraction mediated
by AT 1 receptor activation by AngII. The irst involves Gq and ultimately leads to
MLCK activation. The second proceeds through G 12/13 , the activation of RhoK and the
phosphorylation of MLC. In (b), (c) and (d) are presented the temporal mechanical
responses to AngII after pretreatment with inhibitor targeting directly the actomyosin
contraction (100 μM blebbistatin), MLCK (20 μM ML-9) and RhoK (10 μM Y-27632),
respectively. Scale bars are 50 μm. Reprinted with permission from Ref. 23.
17.5 APPLICABILITY OF AFMBASED MEASUREMENTS TO
OTHER RECEPTOR/AGONIST SYSTEMS
AFM-based force measurement of cell receptor stimulation in living individual
cells produces a robust signal that should allow for label-free detection of a