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(a)
(b)
(c)
(d)
Figure 12.5.
Results of
-actin mRNA mapping in live cells. mRNA was retrieved from
four different sites (inset labels A, B, C and D in each igure) of live cells and ampliied
through quantitative RT-PCR and PCR procedures. Cells in (a) and (b) are in inactive
state with no nutrients in the culture medium, while those in (c) and (d) are active
cells moving in the direction of inset (A). Reproduced with permission from Uehara
et al.
73
B
(a)
(b)
Figure 12.6.
AFM-based quantitation of
-actin mRNA and luorescence
in situ
hybridization. Results from two cells (a) and (b) are listed in the table at the bottom
giving the number of mRNA from four sites. Dark colour in the igures represents
areas where the results of
in situ
hybridization was highly positive. Reproduced with
permission from Uehara
B
et al.
75
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